全文获取类型
收费全文 | 15020篇 |
免费 | 954篇 |
国内免费 | 1075篇 |
出版年
2023年 | 172篇 |
2022年 | 211篇 |
2021年 | 344篇 |
2020年 | 347篇 |
2019年 | 407篇 |
2018年 | 372篇 |
2017年 | 407篇 |
2016年 | 446篇 |
2015年 | 549篇 |
2014年 | 661篇 |
2013年 | 798篇 |
2012年 | 568篇 |
2011年 | 632篇 |
2010年 | 602篇 |
2009年 | 830篇 |
2008年 | 821篇 |
2007年 | 907篇 |
2006年 | 679篇 |
2005年 | 637篇 |
2004年 | 527篇 |
2003年 | 477篇 |
2002年 | 403篇 |
2001年 | 401篇 |
2000年 | 415篇 |
1999年 | 429篇 |
1998年 | 359篇 |
1997年 | 341篇 |
1996年 | 300篇 |
1995年 | 351篇 |
1994年 | 278篇 |
1993年 | 269篇 |
1992年 | 280篇 |
1991年 | 228篇 |
1990年 | 181篇 |
1989年 | 180篇 |
1988年 | 144篇 |
1987年 | 172篇 |
1986年 | 130篇 |
1985年 | 128篇 |
1984年 | 106篇 |
1983年 | 59篇 |
1982年 | 86篇 |
1981年 | 81篇 |
1980年 | 78篇 |
1979年 | 55篇 |
1978年 | 58篇 |
1977年 | 29篇 |
1976年 | 34篇 |
1975年 | 22篇 |
1973年 | 22篇 |
排序方式: 共有10000条查询结果,搜索用时 84 毫秒
41.
Torbjrg Bjelland 《植被学杂志》2003,14(4):525-534
Abstract. The effects of vegetation cover, radiation, micro‐habitat variables and maritime influence on the floristic composition of a saxicolous community in Vingen, western Norway were studied. Particular emphasis is put on the local distribution of Fuscidea cyathoides, Ochrolechia tartarea, Ophioparma ventosa and Pertusaria corallina. Very little of the variation in the lichen community composition is directly related to measured micro‐environmental variables but variance partitioning shows that vegetation cover explains more of the floristic variation than radiation, maritime influence and microhabitat variables. Logistic regression analyses nevertheless indicate that the micro‐environment influences the spatial distribution of the four species. The high fraction of unexplained floristic variation, 91%, is suggested to result from (1) lack of fit of data to the response model; (2) some influential environmental variables that have not been recorded; (3) local historical factors that affect present day distribution and/or (4) apparent randomness in colonization. The results also agree with the view that the four lichen species in this study are able to co‐exist in the long‐term because of different spatial distributions resulting from different strategies with respect to ecology, dispersion and interaction. 相似文献
42.
Nayoung Kang Ah Hyun Jun Yangzom Doma Bhutia Natarajan Kannan Jashvant D. Unadkat Rajgopal Govindarajan 《The Journal of biological chemistry》2010,285(36):28343-28352
Accumulating evidence reveals that sole mutations in hENT3 cause a spectrum of human genetic disorders. Among these include H syndrome, characterized by scleroderma, hyperpigmentation, hypertrichosis, hepatomegaly, cardiac abnormalities and musculoskeletal deformities, pigmented hypertrichotic dermatosis with insulin-dependent diabetes syndrome, characterized by autoantibody-negative diabetes mellitus and skin deformities, familial Rosai-Dorfman disease, characterized by short stature, familial histiocytosis and sinus histiocytosis with massive lymphadenopathy (SHML), characterized by severe tissue infiltration of immune cells and swollen lymph nodes. hENT3 spectrum disorders share a common mutation and share overlapping clinical manifestations that display many intriguing resemblances to mitochondrial and lysosomal disorders. Although earlier studies identify hENT3 as a mitochondrial and a lysosomal nucleoside transporter, the precise connections between hENT3 and the pathophysiology of these disorders remain unresolved. In this study, we performed functional and biochemical characterization of these mutations in hENT3. We report severe reductions/losses of hENT3 nucleoside transport functions of hENT3 syndrome mutants. In addition to transport alterations, we provide evidence for possible loss of hENT3 functions in all H and pigmented hypertrichotic dermatosis with insulin-dependent diabetes syndromes due to either mistrafficking or altered stability of mutant hENT3 proteins. 相似文献
43.
44.
45.
T. J. Orton 《Plant Cell, Tissue and Organ Culture》1985,4(2):159-169
Genetically marked tissues of celery (Apium graveolens) were employed to contrast genetic and chromosomal stability in serially bulk-transferred callus and regenerated plants. After six months in culture, 84% of the callus cells were karologically indistinguishable from normal, while the remainder exhibited chromosome loss and/or fusion. All of 50 clones derived from this tissue expressed the control phenotype with respect to heterozygous isozyme markers. Of 95 plants regenerated from the same tissue, 94 were phenotypically indistinguishable from the original explant donor, and cytogenetic analyses revealed the presence in 4.3% of an accessory chromosome, while the remainder were normal diploids. Analysis of the selfed progeny of these regenerated plants revealed the presence of a new recessive mutation causing abnormal leaf morphology at a frequency of 1.8%. Only one of 40 cells in 12-month-old callus tissue was karyologically indistinguishable from normal, the remainder consisting primarily of hypodiploids. The observation that all 50 clones were phenotypically heterozygous was statistically inconsistent with the hypothesis that hypodiploidy was associated with random complete chromosome loss. The culture had, at this point, lost the ability to regenerate. It is speculated that embryogenic cloning of celery may be suitable under certain circumstances for direct field establishment, but that levels of new genetic variation are sufficiently high to preclude its use for seed production. 相似文献
46.
Margaret J. Davies Joanne G. Turner Cristofol Vives-Bauza Paul C. Rumsby 《Mutation research》1999,431(2):558
In an attempt to understand the inter-individual variation that occurs in in vivo mutant frequency at the HPRT locus, we have examined the effect of polymorphisms in genes for metabolic enzymes on the mutation rate. In the same population of human volunteers, the background variant frequency in a number of microsatellite sequences was studied to determine individual variation in the capacity to repair mismatches in these sequences. The HPRT mutant frequency of T-cells isolated from a group of 49 healthy, non-smoking adults varied from 0.25 to 9.64×10−6. The frequency of polymorphisms in CYP1A1, GSTM1 and NAT2 among these individuals was similar to those published, and when subjected to univariate analysis these polymorphisms showed no influence on the HPRT mutant frequency. However, there was a significant interaction between the GSTM1 null genotype and the slow acetylator status in NAT2 (P<0.05) which was associated with higher mutant frequency. Analysis of 30 microsatellite sequences in 20 HPRT proficient clones per individual showed only six alterations in total, giving an overall mutation rate per allele of 0.01%, whilst three alterations were found in five HPRT deficient clones per individual examined for changes in 10 microsatellites, giving an overall mutation rate per allele of 0.3%. Thus, the alterations detected are probably due to background mutations and not to differences in mismatch repair capacity. 相似文献
47.
Marion S. R?der Mark E. Sorrells Steven D. Tanksley 《Molecular & general genetics : MGG》1992,232(2):215-220
Summary The long-range structure of 5S rRNA gene clusters has been investigated in wheat (Triticum aestivum L.) by means of pulsed field gel electrophoresis. Using aneuploid stocks, 5S rRNA gene clusters were assigned to sites on chromosomes 1B, 1D, 513 and 5D. Cluster sizes were evaluated and the copy number of 5S DNA repeats was estimated at 4700-5200 copies for the short repeating unit (410 bp) and about 3100 copies for the long repeat (500 bp) per haploid genome. A comparison of wheat cultivars revealed extremely high levels of polymorphism in the 5S rRNA gene clusters. With one restriction enzyme digest all varieties tested gave unique banding patterns and, on a per fragment basis, 21-fold more polymorphism was detected among cultivars for 5S DNA compared to standard restriction fragment length polymorphisms (RFLPs) detected with single copy clones. Experiments with aneuploid stocks suggest that the 5S rRNA gene clusters at several chromosomal sites contribute to this polymorphism. A number of previous reports have shown that wheat cultivars are not easily distinguished by isozymes or RFLPs. The high level of variation detected in 5S rRNA gene clusters therefore offers the possibility of a sensitive fingerprinting method for wheat. 5S DNA and other macro-satellite sequences may also serve as hypervariable Mendelian markers for genetic and breeding experiments in wheat. 相似文献
48.
Heterochrony, an evolutionary change in developmental processes, is one of the major proximate causes of morphological diversity of organisms. It has been reported in the medaka Oryzias latipes that higher-latitude larvae have a genetic tendency to complete fin ray formation at larger body sizes, which results in relatively shorter anal and dorsal fins in adults. However, this latitudinal, heterochronic variation in fin length in the wild may be partially explained by latitudinal differences in thermal environments, if temperatures affect the timing of fin ray formation. Common-environment experiments revealed that the body size at which fin pterygiophore (a basal skeleton of fin rays) formation was completed was larger in higher-latitude larvae than in lower-latitude larvae at all temperatures examined, supporting the proposal that fin ray formation of the former is genetically delayed. However, phenotypic plasticity in response to temperature was also evident; lower temperatures caused delayed fin ray formation until a larger body size had been achieved in both high- and low-latitude larvae. These observations suggest that habitat temperatures also contribute to the latitudinal difference in the timing of fin development, magnifying phenotypic variation in fin length across latitudes. We discuss reasons for this positive covariance between genetic and environmental effects on the latitudinal, heterochronic variation, from the viewpoint of local adaptation and evolution of phenotypic plasticity. 相似文献
49.
FIONA J. QUALLS RICHARD SHINE 《Biological journal of the Linnean Society. Linnean Society of London》2000,71(2):315-341
While recent experimental work on a variety of reptile species has demonstrated that incubation temperature influences hatchling phenotypes, the biological significance of such phenotypic variation remains unclear. Incubation temperature may exert significant long-term phenotypic effects. Alternatively, such influences may be temporary, or negligible relative to effects induced by genetic factors, or by the environmental conditions experienced after hatching. Even if incubation temperature exerts long-term effects on phenotype, this might occur indirectly (by influencing hatching dates) rather than by direct modifications of developmental processes. We quantified the influences of the source population, incubation temperature and rearing environment, on the phenotype of the Australian garden skink (Lampropholis guichenoti) from populations that differ in nest temperature and phenotype. Intcrpopulation differences in the phenotypes of young lizards were found to be a product of all three factors. However, the long-term effects of both population and incubation temperature operated indirectly (through variation in the date of hatching) rather than directly (through genetic or developmental factors). That is, once all temporal effects were removed, the only discernible influence on juvenile phenotypes was their rearing environment. Thus, some of the most important influences on lizard phenotypes may operate via modifications of hatching date. 相似文献
50.
Recruitment of scleractinian corals on settlement plates at Heron Island, Great Barrier Reef, was examined over four years
(September 1991–September 1995) to quantify spatio-temporal patterns at different scales and to assess post settlement mortality.
Recruitment was dominated by pocilloporid corals which accounted for 80.1% of the 8627 spat counted, whereas non-isoporan
acroporids represented only 16.4%. Poritids, faviids and isoporan acroporids rarely recruited to the plates (3.5%), despite
their obvious abundance as adults on the reef. Recruitment patterns on the plates indicate strong space-time interactions
as evidenced by patchy recruitment of both pocilloporid and acroporid spat. Interactions were found between space (on the
scale of 102 m, i.e. sites within zones, and 101 m, i.e. racks within sites) and time (on the scale of years) for pocilloporids and between space (on the scale of 103 m, i.e. zones, and 102 m) and time (on the scale of years) for acroporids. Post-recruitment mortality of acroporid spat in the period 3–10 months
after their major spawning was dependent on their initial recruitment density, but pocilloporid mortality was either independent
of initial recruitment density or, more likely, obscured by additional recruitment of pocilloporids to plates between late
February and September. High rates of recruitment and growth by other sessile organisms, particularly bryozoans and oysters,
appear to result in increased post-recruitment mortality and limit recruitment of scleractinian corals on settlement plates.
The work reinforces an emerging picture that coral recruitment patterns are determined by mechanisms that manifest over a
large range of spatial scales.
Accepted: 1 September 1997 相似文献